Preskoči na vsebino
Loader

MEDIA CENTER

ALL NEWS

Categories

Date

  • 30. Nov 2021

Share with others

Study of gold nanoparticles’ preparation through ultrasonic spray pyrolysis and lyophilisation for possible use as markers in LFIA tests

To monitor the progress and prevent the spread of theCOVID-19 pandemic in real time and outside laboratories, it is essential todevelop effective tests that can ensure rapid, selective, and reliablediagnosis of infected persons in different environments. Key in this regard isthe lateral flow ​immunoassays (LFIAs) that can detect the presence ofthe SARS-CoV-2 virus quickly, with the aid of nanoparticles (NPs) and specificproteins.

Slika6

To monitor the progress and prevent the spread of theCOVID-19 pandemic in real time and outside laboratories, it is essential todevelop effective tests that can ensure rapid, selective, and reliablediagnosis of infected persons in different environments. Key in this regard isthe lateral flow ​immunoassays (LFIAs) that can detect the presence ofthe SARS-CoV-2 virus quickly, with the aid of nanoparticles (NPs) and specificproteins.

We report the use of gold (Au) NPs AuNPs synthesised from a gold(iii)chloride tetrahydrate precursor in a USP device and collected in a suspensioncomposed of deionised water with polyvinylpyrrolidone as a stabiliser andcryoprotectant. In combination with freeze-drying of the AuNPs’ suspension toachieve water elimination, improved stability, and the target concentration,they exhibit the necessary properties for use as markers in LFIA rapiddiagnostic tests. This was confirmed by complementary characterisationdetermined by using the techniques including inductively coupled plasma-opticalemission spectrometry, dynamic light scattering method and zeta-potential,ultraviolet-visible spectroscopy, X-ray diffraction, scanning electronmicroscopy with energy dispersion spectrometer, and transmission electronmicroscopy, as well as with the preparation of a prototype LFIA test strip withAuNPs. Thus, such AuNPs, as well as the USP method, show promise for thedevelopment of new markers for use in LFIA.

https://www.degruyter.com/document/doi/10.1515/ntrev-2021-0120/html 

 


Categories

Date

  • 30. Nov 2021

Share with others

More news

Close